The Following changes in ECS require a reset of all talk and listen paths (Black Reset)

Panel change GN Microphone gain (Matrix Hardware screen)V-Series Panel change HS1 Microphone gain  (Matrix Hardware screen) V-Series Panel change HS2 Microphone gain  (Matrix Hardware screen) Reset all crosspoints to ECS settingsRemove all locally assigned keysDeactivate all panel keysReset Panel listen levels set to normalLocal panel microphone settings reset to the ECS downloaded value.Reset local changes to groups, party lines and IFBs made via ICS2003, V-series and i-stations to ECS valuesReset Matrix Input levels - Matrix input levels are set to the ECS download value.Reset Matrix Output levels - Matrix output levels are set to the ECS download value.
To forced a black reset download to the frame: Select apply changes > select options > select Clear Frame data.